Determination of the Antibacterial Activity of different Toothpastes against Pathogenic Bacteria Isolated from Tooth Gums
Keywords:
S. aureus , M. Kristnae, Cellobiosococcus Spp, K. Pneumoniae, Antibacterial ActivityAbstract
OBJECTIVES
This study aimed to determine the anti-bacterial activity of different toothpastes against the pathogenic bacteria isolated from gums.
METHODOLOGY
For this study, 100 gum samples were collected from dental clinics in Peshawar using sterile disposal swabs. The samples were transported to Peshawar’s Abasyn University and streaked on Nutrient Agar plates. The obtained cultures were sub-cultured and processed for further identification by Gram staining and biochemical tests.
RESULTS
It was found that, out of a total of 100 samples, 60 were positive (40 males and 20 females), and 40 were negative. Among 60 samples, 9 species were identified, and both female and male samples showed a high prevalence of S. aureus. After the analysis of all 5 toothpastes’ anti-bacterial activities, it was observed that all toothpastes showed activity against bacteria except toothpaste number 4. Those toothpastes that showed maximum activity were enlisted: TP-1 [Cellobiosococcus spp. (16.6 ± 0.57)], TP-2 [M. kristnae (16.6 ± 0.57)], TP-3 [M. mucilaginous (15.5 ± 0.86)], and TP-5 [K. pneumoniae (15.5 ± 0.86)].
CONCLUSION
The outcome of the studies concluded that the prevalence of bacteria isolated from male samples was higher and more pathogenic than in female samples.
References
Findley K, Grice EA. The skin microbiome: a focus on pathogens and their association with skin disease. Plos pathogens. 2014 Nov 13;10(11):e1004436
Ritchie KB, Schwarz M, Mueller J, Lapacek VA, Merselis D, Walsh CJ, Luer CA. Survey of antibiotic-producing bacteria associated with the epidermal mucus layers of rays and skates. Frontiers in microbiology. 2017 Jul 5;8:1050
Shenoy PA, Vishwanath S, Gawda A, Shetty S, Anegundi R, Varma M, Mukhopadhyay C, Chawla K. Anaerobic bacteria in clinical specimens–frequent, but a neglected lot: a five year experience at a tertiary care hospital. Journal of clinical and diagnostic research: JCDR. 2017 Jul;11(7):DC44.
Temple A, Riley H, Ramachandran A, Hoff W. Pan Genomes of Pathogenic Bacteria. Research Reports from Life Science Freshmen Research Scholars. 2017 Oct 4;3(1).
Orji FA, Ugbogu OC, Ugbogu EA, Barbabosa-Pliego A, Monroy JC, Elghandour MM, Salem AZ. Pathogenic flora composition and overview of the trends used for bacterial pathogenicity identifications. Microbial pathogenesis. 2018 Aug 1;121:139-46.
Shahcheraghi SH, Ayatollahi J, Lotfi M, Bafghi AF, Khaleghinejad SH. Application of nano drugs in treatment of leishmaniasis. Glob J Infect Dis Clin Res 2 (1): 018-020. DOI: 10.17352/2455. 2016;5363(018):1-5.
Li F, Tao D, Feng X, Wong MC, Lu H. Establishment and development of oral microflora in 12–24 month-old toddlers monitored by high-throughput sequencing. Frontiers in cellular and infection microbiology. 2018 Dec 4;8:422.
Chawla R, Shetty K, Prakash A, Rathore A, Saroch SJ. Orthodontics and oral microflora: synergism or parasitism. J Interdiscipl Med Dent Sci. 2018;6(234):2-5.
Okafor AC, Igwesi SN, David EE, Okolo VK, Agu K. Presence of bacteria with pathogenic potential among already-used toothbrushes from university students. American Journal of Life Science Researches. 2016 Jul 24;4(3).
Hajishengallis E, Parsaei Y, Klein MI, Koo H. Advances in the microbial etiology and pathogenesis of early childhood caries. Molecular oral microbiology. 2017 Feb;32(1):24-34
Bhattacharjee S, Nath S, Bhattacharjee P, Chouhan M, Deb B. Efficacy of toothpastes on bacteria isolated from oral cavity. International Journal of Medicine and Public Health. 2018;8(2)
Froböse NJ, Bjedov S, Schuler F, Kahl BC, Kampmeier S, Schaumburg F. Gram staining: a comparison of two automated systems and manual staining. Journal of Clinical Microbiology. 2020 Nov 18;58(12):e01914-20
Shoaib M, Muzammil I, Hammad M, Bhutta ZA, Yaseen I. A mini-review on commonly used biochemical tests for identification of bacteria. A Mini-Review on Commonly used Biochemical Tests for Identification of Bacteria. 2020 Jun 14;54(1):8-
Christensen JJ, Ruoff KL. Aerococcus, Abiotrophia, and other aerobic catalase‐negative, Gram‐positive cocci. Manual of clinical microbiology. 2015 May 15:422-36.
Rajan BM, Kannabiran K. Extraction and identification of antibacterial secondary metabolites from marine Streptomyces sp. VITBRK2. International journal of molecular and cellular medicine. 2014;3(3):130.
Kavitha K, Ragunathan L, Elantheriyan P, Gopalakrishnan K, Gopala K, Balamurugan I, Navya R, Marcella S, Venkatachalam G. The burden of mycobacteria species among children from postvaccination abscess in Southern India. International Journal of Mycobacteriology. 2021 Oct 1;10(4):358-
Albadri AT. Characterization and Molecular study to detect Multidrug Resistance Bacteria Isolated from Patients with Diabetic Foot Ulcers in Wasit Province (Doctoral dissertation, Ministry of Higher Education) (Doctoral dissertation, M. sc Thesis, Wasit University, Wasit, Iraq).
Kafilzadeh F, Dehdari F. Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of Iran. The Egyptian Journal of Aquatic Research. 2015 Jan 1;41(2):197-201
Bouyahya A, Abrini J, El-Baabou A, Bakri Y, Dakka N. Determination of phenol content and antibacterial activity of five medicinal plants ethanolic extracts from North-West of Morocco. J Plant Pathol Microbiol. 2016;7(342):2
Bibi K, Rehman A, Fatima A, Ali G, Ishaq MS, Samad A, Asim M, Ullah S, Siddiqui MF. Assessment of antibiotics and fluoride toothpastes against pathogenic bacteria associated with dental caries. Abasyn Journal of Life Sciences. 2018;1(1):1-0
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2023-06-01
