Determination of Antibacterial Activity of Nicotiana Tabacum Against Bacteria Isolated From Teeth Gums of Smokers and Non Smokers
Keywords:
S. Aureus, M. Mucilaginous, E. Coli, S. Hyicus, S. Cohnii, Antibacterial ActivityAbstract
OBJECTIVES
This study aimed to determine the antibacterial activity of Nicotiana tabacum against bacteria isolated from the gums of smokers and non-smokers.
METHODOLOGY
For this study, 100 gum samples were collected from dental clinics in Peshawar using sterile disposal swabs. The samples were transferred to Abasyn University in Peshawar, streaked on Nutrient agar plates. The obtained cultures were sub-cultured and processed for further identification by Gram staining and biochemical tests.
RESULTS
It was found that, out of a total of 100 samples, 60 were positive (35 non-smokers and 25 smokers), and 40 were negative. Among 60 samples, 12 species were identified, of which M. mucilaginous (24% of the smoker) and S. aureus (44% of the non-smoker) showed high prevalence. After the analysis of both the dried and chewed N. tabacum’s antibacterial activity, it was observed that dried tobacco extract showed maximum activity against S. hyicus (16.33 ± 0.57), M. mucilaginous (16.33 ± 0.57) and least activity against E. coli (10.7 ± 0.46). In contrast, chewed tobacco extract showed maximum activity against S. cohnii (15.33 ± 0.57), while the remaining isolates were resistant.
CONCLUSION
The outcome of the studies concluded that the prevalence of bacteria isolated from smoker's samples was higher and more pathogenic than in non-smoker’s samples.
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2022-12-05
