Levels of Vitamin B12 and Homocysteine in Pre-Eclamptic Patients with and Without Placenta Abruption


  • Firdous Jamil Khyber Medical University Peshawar
  • Muhammad saeed IBMS, KMU, Peshawar
  • Maria Karim IBMS, KMU, Peshawar
  • Tahoor Khan IBMS, KMU, Peshawar


Preeclampsia, Vitamin B12, Homocysteine, Placenta abruption



This study aimed to determine vitamin B12 and homocysteine levels in pre-eclamptic patients with and without placenta abruption.


A case-control study comprised 60 participants (cases n =30 and control n = 30) ages 17-28 years to evaluate the correlation between vitamin B12 and homocysteine. Each participant was interviewed for data collection regarding their age, education, income, gestation weeks, and family history of hypertension and their blood pressure, urinary albumin, height, weight, and body mass index in kg/m2 (BMI). Blood samples (5 ml) were collected from each subject through sterilized syringes; 3 ml of blood was transferred to the ETDA tube and 2 ml into the clot activator Gel tube. Each sample was centrifuged and stored at -20C. Vitamin B12 and homocysteine ELISA kits were used for measuring the vitamin B12 as well as homocysteine levels in each blood sample. Vitamin B12 was analyzed by Chemiluminescent Microparticle Intrinsic Factor assay. For both Vitamin B12 and homocysteine analysis, a commercially available ARCHITECH kit was used. SPSS version 26.0 was used.


It was observed that vitamin B12 in both cases and control were the most frequent. In case twenty-three (77%) and control twenty-two (73%) are in the normal range. Five (17%) and seven (23%) are deficient in both case and control, respectively. Only two (7%) and one (3%) had a high vitamin b12 in the case and control, respectively. On the other hand, none of the cases was deficient with homocysteine, while only three (3%) in control were deficient with homocysteine. Three (10%) and six (20%) had high homocysteine levels than normal in the case and control, respectively.


Our results highlight the importance of Vitamin B12 and homocysteine in pregnancy. Further large-scale studies should evaluate the relationship between Vitamin B12 and homocysteine in pre-eclampsia and abruption.

Author Biographies

Firdous Jamil, Khyber Medical University Peshawar

Student, Biochemistry,

Khyber Medical University, Peshawar

Muhammad saeed, IBMS, KMU, Peshawar

M.Phil Scholar, Biochemistry,
IBMS, KMU, Peshawar

Maria Karim, IBMS, KMU, Peshawar

M.Phil Scholar, Biochemistry,

IBMS, KMU, Peshawar

Tahoor Khan, IBMS, KMU, Peshawar

M.Phil Scholar, Biochemistry,

IBMS, KMU, Peshawar


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How to Cite

Jamil, F. ., Muhammad saeed, Karim, M. ., & Khan, T. . (2022). Levels of Vitamin B12 and Homocysteine in Pre-Eclamptic Patients with and Without Placenta Abruption. Journal of Wazir Muhammad Institute of Paramedical Technology, 2(2), 41–46. Retrieved from https://jwmipt.org.pk/index.php/JWMIPT/article/view/48